Messi is one of five players suspended for the visit to Bolivia after Nicolas Otamendi, Javier Mascherano, Lucas Biglia and Gonzalo Higuain picked up yellow cards against Chile.
To compound coach Edgardo Bauza's selection headache, Gabriel Mercado and Emmanuel Mas both suffered injuries in that match.
More encouraging for Argentina, however, is the fact that Bolivia have lost 10 of their 13 qualifiers so far despite their well-documented altitude advantage at Estadio Hernando Siles, which is one of the highest stadiums in the world at 3,637 metres above sea level.